Protein Expression

Aragen Bioscience produces proteins at the mg scale up to gram quantities for antibodies, recombinant enzymes and other formats in a range of platforms including:

Mammalian Expression

  • Transiently transfection in:
    • HEK-293 cells
    • CHO-S cells
  • Stable cell lines
  • Hybridomas

E. coli Expression

Protein Purification

Aragen has experience in the purification of secreted monoclonal antibodies, intracellular tagged proteins, novel cytokine fusion molecules as well as large, complex proteins produced by a variety of expression platforms:

Purification of:

  • fusion and tagged proteins from recombinant sources.
  • native or untagged proteins.
  • purification and refolding from inclusion bodies.

Aragen’s ÄKTA-based purification systems can accommodate lots in the range of ~100 μg to 25 – 100g with feedstock volumes matching our upstream cell culture capacity of up to ~100L.

Early to late Discovery

Our team will work closely with you from concept through delivery. Together, we’ll develop a set of project deliverables tailored to your specifications of scale, purity, activity and other analytical characteristics on a timeline that meets your needs.

The protein science group works in close collaboration with Aragen’s cell biology and in vivo teams to provide a tightly integrated solution for your development needs.

Ready to take the next step?