Fibrosis Models

Aragen Bioscience has a portfolio of in vitro and in vivo fibrosis models and world-class expertise to support you during the development of your anti-fibrotic therapeutic or prophylactic.  In hundreds of studies over the course of a decade, we have evaluated the efficacy of test compounds spanning several modalities (small molecules, biologics, RNA etc.) in a variety of assay platforms, ultimately resulting in multiple INDs for our clients.

As the most commonly used model for IPF, Bleomycin induction causes inflammatory and fibrotic reactions in rodent lungs within a short period of time and successfully replicates many of the pathological characteristics, including abnormal deposition and accumulation of collagen in lung tissue, associated with IPF. Learn more about our lung fibrosis models. 

This rodent model, induced by the administration of crystalline silico dioxide (or silica) displays many pathophysiological features of chronic inflammation and pulmonary fibrosis, which can be used as an experimental model for IPF and silicosis.  Learn more about our lung fibrosis models. 

CCL4 induced liver fibrosis
In this model, the hepatotoxicity of carbon tetrachloride (CCl4) induces liver injury and liver fibrosis within a short period of time, which consequently can lead to liver cirrhosis and hepatocellular carcinoma (HCC).

As a second extensively used model in rodents, the thioacetamide (TAA) administration reproducibly induces chronic liver injury and fibrosis, and progression of liver cirrhosis.

In this model, animals are fed a choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD). In a short period of time CDAHFD feeding results in the induction of pathophysiological characteristics of NASH, such as hepatic lipid accumulation, hepatic inflammation, and liver injury, and rapid progression of liver fibrosis and hepatocellular carcinoma (HCC).

Through feeding a western diet (WD), which is high-fat, high-fructose and high-cholesterol, and weekly administering low doses of carbon tetrachloride (CCl4), this model shows the induction of pathophysiological features of NASH and rapid progression of liver fibrosis and hepatocellular carcinoma (HCC).

By feeding the AMLN (amylin liver NASH) diet high in trans-fat, fructose and cholesterol, this model can successfully display many pathophysiological features of NASH as well as metabolic syndrome.

Bleomycin induced systemic sclerosis model (lung and skin fibrosis)

With subcutaneous injection of bleomycin, this model develops skin and pulmonary inflammation and fibrosis, which can be used as a model for human systemic sclerosis.

Fibrosis Technical Aid

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