Protein Analytics

Aragen Bioscience, a leading CRO, offers a range of analytical methods utilizing multiple assay platforms that be used to characterize a broad range of proteins expressed and purified by various methods. We take a wholistic approach to protein characterization and will work with to develop a set of studies that will help you reach your research or development goals. 

  • Protein activity
    • Binding affinity (FortéBio Octet® RED96 System, GE Biacore™ 3000)
    • Enzyme activity
    • Cell-based assays
    • In vivo testing

     

  • Protein stability (Unchained Labs Uncle)
    • Tm & Tagg
    • ΔG
    • Isothermal stability
    • Thermal recovery
    • Sizing & polydispersity
    • Sizing with thermal ramp
    • B22 & kD
    • Viscosity

     

  • Protein titer/concentration
    • Protein A HPLC
    • ELISA
    • A280
  • Protein integrity and structure
    • Size variants by SEC-HPLC
    • Size variants by SEC-MALS
    • Size variants by CE-SDS (reduced and non-reduced)
    • Hydrophobic variants by RP-HPLC/UPLC or HIC
    • Protein purity by SDS-PAGE
    • N-glycan analysis by HILIC-FLD
    • N-glycan analysis by CE
    • Sialic acid content by DMB RP
    • Free thiol content by SEC-FLD
    • Charge variants by cIEF
    • Western Blot
    • HCP titer by ELISA
    • Residual Protein A, Protein G, Protein L by ELISA or Octet
    • Endotoxin levels by LAL

     

  • Protein mass (Agilent HPLC-CHIP/QTOF)
    • Accurate mass: proteins, protein-drug conjugates
    • Peptide and Glycan mapping
    • Determination of post-translations modifications either in known or unknown samples.
    • Standard COAs

Our team will work closely with you from concept through delivery. Together, we’ll develop a set of project deliverables tailored to your specifications of scale, purity, activity and other analytical characteristics on a timeline that meets your needs. The protein science group works in close collaboration with Aragen’s cell line development and pre-clinical efficacy testing teams to provide a tightly integrated solution for your development needs.

Ready to take the next step?