Protein Analytics

Aragen Bioscience, a leading CRO, offers a range of analytical methods utilizing multiple assay platforms that be used to characterize a broad range of proteins expressed and purified by various methods:

Protein activity
- Binding affinity (FortéBio Octet^® RED96 System)
- Enzyme activity
- Cell-based assays
- In vivo testing
Aggregation status
- SDS-PAGE
- SEC-HPLC
- Optical density

Protein stability (Unchained Labs Uncle)
- T_m & T_agg
- ΔG
- Isothermal stability
- Thermal recovery
- Sizing & polydispersity
- Sizing with thermal ramp
- B₂₂ & k_D
- Viscosity

Protein integrity and structure
- - SEC-HPLC
  - SDS-PAGE
  - N-glycan analysis
  - Western Blot
Protein mass (Agilent HPLC-CHIP/QTOF)
- - Accurate mass: proteins, protein-drug conjugates
  - Peptide and Glycan mapping
  - Determination of post-translations modifications either in known or unknown samples.
  - Standard COAs

Our team will work closely with you from concept through delivery. Together, we’ll develop a set of project deliverables tailored to your specifications of scale, purity, activity and other analytical characteristics on a timeline that meets your needs. The protein science group works in close collaboration with Aragen’s cell biology and in vivo teams to provide a tightly integrated solution for your development needs.

Ready to take the next step?