Protein Analytics
Aragen Bioscience, a leading CRO, offers a range of analytical methods utilizing multiple assay platforms that be used to characterize a broad range of proteins expressed and purified by various methods. We take a wholistic approach to protein characterization and will work with to develop a set of studies that will help you reach your research or development goals.
- Protein activity
- Binding affinity (FortéBio Octet® RED96 System, GE Biacore™ 3000)
- Enzyme activity
- Cell-based assays
- In vivo testing
- Protein stability (Unchained Labs Uncle)
- Tm & Tagg
- ΔG
- Isothermal stability
- Thermal recovery
- Sizing & polydispersity
- Sizing with thermal ramp
- B22 & kD
- Viscosity
- Protein titer/concentration
- Protein A HPLC
- ELISA
- A280
- Protein integrity and structure
- Size variants by SEC-HPLC
- Size variants by SEC-MALS
- Size variants by CE-SDS (reduced and non-reduced)
- Hydrophobic variants by RP-HPLC/UPLC or HIC
- Protein purity by SDS-PAGE
- N-glycan analysis by HILIC-FLD
- N-glycan analysis by CE
- Sialic acid content by DMB RP
- Free thiol content by SEC-FLD
- Charge variants by cIEF
- Western Blot
- HCP titer by ELISA
- Residual Protein A, Protein G, Protein L by ELISA or Octet
- Endotoxin levels by LAL
- Protein mass (Agilent HPLC-CHIP/QTOF)
- Accurate mass: proteins, protein-drug conjugates
- Peptide and Glycan mapping
- Determination of post-translations modifications either in known or unknown samples.
- Standard COAs
Our team will work closely with you from concept through delivery. Together, we’ll develop a set of project deliverables tailored to your specifications of scale, purity, activity and other analytical characteristics on a timeline that meets your needs. The protein science group works in close collaboration with Aragen’s cell line development and pre-clinical efficacy testing teams to provide a tightly integrated solution for your development needs.
Ready to take the next step?