April 8-12, 2019, Boston, MA
We are excited for this year’s PEGS Boston Summit! Visit booth 427 and discover how Aragen Bioscience can move your pre-clinical research projects forward. Don’t miss our poster (Poster Session A, Monday/Tuesday)!
Resolving Precipitation Issues of an Antibody-Fusion During Protein A Purification Through Flocculation
Rene Pagila*, Michelle Stroud and Tim Myles
Two distinct antibody-fusion proteins that share a common fusion partner expressed well in a transient CHO system, however, both have the propensity to precipitate upon elution during Protein A purification. Employing flocculation on the Protein A starting material resolved the precipitation issue.