Protein Chemistry Service:

Aragen’s scientists offer a wide range of expertise in a variety of protein chemistry analytical methods. Many of these services are listed below.

1. Bio-conjugation

   1. Protein – protein
   2. Protein – peptide
   3. Protein – small molecule
   4. Protein - polymer (PEG etc.)
   5. Protein – resins

2. Protein engineering

   1. Design of construct
   2. Mutation/isoform/ortholog

3. Protein expression system screening

   1. Protein yield
   2. Protein solubility
   3. Protein stability
   4. Post-translational modifications

4. Characterization

   1. Protein identification
      1. SDS-PAGE, IEF, Native, Western Blot
      2. N-terminal sequence
      3. Peptide mapping
      4. MALDI-TOF, ESI-MSMS
   2. Protein activity
      1. Catalytic activity
      2. Binding activity (Steady state, kinetic)
      3. Inhibition (IC50, Ki)
   3. Post-translation
      1. Glycosylation
      2. Phosphorylation, etc.
   4. Protein activation
   5. Proteolytic activation
   6. Chemical activation
   7. Phosphorylation

5. Protein formulation

   1. Solvent screening
   2. Forced degradation
   3. Thermostability (Tm)
   4. Aggregation kinetics
   5. Concentration limit

6. Biopharma product lot release assay

   1. LAL endotoxin
   2. Host cell residual proteins
   3. Host cell residual DNA
   4. Residual Protein A