In Vivo Efficacy Testing Services

“Aragen Biosciences is a versatile contract research lab that keeps expanding their services. Most recently, they have been a solid performer for my company’s animal studies. This is the second company where I have had the good fortune to work with them and I can say that they keep exceeding expectations. The Aragen team is very good at communicating and they consistently deliver quality results. They do a great job staying on time lines and they make sure that all the details of the project are worked out well in advance. Their flexibility is another thing that really sets them apart from their competitors. They really work with the client to make sure the study is well planned and has the best chance of successfully achieving the goal. They use high quality equipment as well. Recently, we had the good fortune of using their new IVIS® Lumina XRMS system and were delighted with the results. It helped us to rapidly validate one of our new ADCs, and the data correlated quite well with traditional caliper based tumor measurements. We recently were showing a potential collaborator the data and having the images alongside the traditional tumor measurements was game changing for us! We have been so pleased with the work they have done to help validate our molecules in vivo, we are now thinking about working on antibody cell line development with them. Thank you Aragen!” – Elisabeth Gardiner, PhD, Chief Scientific Officer, Meditope Biosciences, Inc

Our highly experienced team will guide you through in vivo testing by recommending the most appropriate animal models, conducting studies with well established protocols and generating reports customized to your needs. We have established a broad range of working animal models that support multiple therapeutic/disease areas.

Animal Models of Disease

In Vivo Expertise and Resources that Drives Outcomes

Our team will help you investigate different animal protocols and optimize planning and experimental design to ensure you get the most out of your in vivo studies. Because of our scale, we can quickly allocate the appropriate space and technical staff to carry out the designed experiments. We provide specialized platforms and technical expertise including ex vivo/in vitro testing and histological preparations. We also undertake non-GLP pharmacokinetic (PK), pharmacodynamics (PD), and toxicology screens.

Our campus includes a recently commissioned state of the art 15,500 square foot facility dedicated to in vivo pharmacology and ex vivo analysis and an additional 16,000 square foot facility for in vitro analysis. These resources, combined with our specialized expertise in animal handling, colony management and small animal procedures, help ensure a successful outcome to your pre-clinical studies.


Technology Focus: IVIS Imaging

One of the most powerful technologies available to assess tumor growth and development, as well as efficacy of candidate therapeutics, is Bioluminescence Imaging. We offer this powerful modality using PerkinElmer’s IVIS® Lumina XRMS system, which includes X-Ray and fluorescence imaging capabilities with precise overlay.

Application Example: IVIS Imaging and Caliper Measurement of Subcutaneous SK-OV-3-Luc Xenograft in Athymic Nude Mice

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2 x 106 SK-OV-3-Luc cells were injected subcutaneously (SC) into the dorsal flank of female athymic nude mice.

Tumor growth was monitored for luciferase expression using the PerkinElmer IVIS at various time points. Mice were imaged 10 minutes post SC injection of luciferin at 150 mg/kg at various time points. IVIS imaging detected as early as Day 10 Post Injection (pi) of tumor growth compared to the caliper measurement.

IVIS is one of the many technology platforms that we employ.


ONCOLOGY MODELS

Human Tumor Xenograft Models

  • Human breast carcinomas: MDA-MB-231, MDA-MB-468, MCF-7, SKBr3, BT-474
  • Human lung carcinomas: A549, A427, NCI-H1993, NCI-H1299, NCI-H226
  • Human lymphoma: My-La, Raji, Daudi
  • Human pancreatic: PANC-1, MIA-PACA, PANC 10.05
  • Human colon carcinomas: HT29, HCT116
  • Human leukemia: Kasumi, KG-1, NCI-H929, RPMI-8266, MM.1S, U266
  • Additional cell lines xenograft models available

Syngeneic Tumor Models

Breast, Colon, Melanoma, Liver, Lung, Lymphoma
Other Oncology Models

  • Lung metastasis model
  • Orthotopic models of breast, kidney
  • Subrenal capsule assay
  • Vaccine model

Visit Oncology Models for details

FIBROTIC DISEASE MODELS

  • Lung fibrosis: Bleomycin-induced lung fibrosis in mice, rats
  • Liver fibrosis: CCl4-induced liver fibrosis in mice
  • Liver fibrosis: TAA-induced liver fibrosis in rats

Visit Fibrotic Disease Models for details.

INFECTIOUS DISEASE MODELS

  • Clostridium difficile associated diarrhea model in hamsters and mice
  • Mouse Cytomegalovirus (mCMV)
  • Respiratory Syncytial Virus (RSV) in mice and cotton rats

Visit Infectious Disease Models for details.

IMMUNOLOGY/INFLAMMATION/AUTOIMMUNE DISEASE MODELS

  • Collagen-induced arthritis: DBA/1 mice, Lewis rats
  • Adjuvant-induced arthritis: rats
  • EAE model: SJL mouse, Lewis rats
  • Lupus: NZBxNZW F1 mouse, MRL/LPR
  • Delayed-type hypersensitivity in mice
  • Type 1 diabetes in mice
  • Anaphylaxis model in mice
  • Endotoxemia models in mice and rats
  • DSS induced IBD model in mice

Visit Immunology/Inflammation Disease Models for details.

RESPIRATORY DISEASE MODELS

  • Asthma model: BALB/c, BDF1
  • Lung fibrosis: C57B/L6, Wistar rats
  • Respiratory Syncytial Virus (RSV): BALB/c and cotton rats

Visit Respiratory Disease Models for details

OTHER MODELS

  • Pericarotid collar implantation model for localized atherosclerosis in mice
  • Thrombosis model: Ferric chloride-induced arterial thrombosis in mice
  • Angiogenesis models: Matrigel plug assay, Trevigen implants in mice
  • Wound healing model: Excisional, and incisional models in normal and diabetic mice
  • Musculo-skeletal syndrome (MSS) model in rats
  • Osteochondral cartilage repair model
  • Custom models of disease
  • PK and Tox Studies

Visit Other Models for details.

Ready to take the next step? Contact us!

The In Vivo Efficacy services performed by Aragen Bioscience are non-GLP studies performed exclusively in rodent models.

Aragen Bioscience has a long tradition of humane and proper use of laboratory research animals for biological and biomedical research. The Institutional Animal Care and Use Committee (IACUC) serves as an independent oversight committee responsible for monitoring all associated animal care and use at Aragen Bioscience.

The presence of IACUC and their responsibility in assuring adherence to federal, state, and institutional guidelines for all animal care and use are monitored by accrediting agencies that include USDA and the Public Health Service/National Institutes of Health/Office of Lab Animal Welfare (OLAW). Aragen Bioscience also holds a Controlled Substance License from the U.S Department of Justice/Drug Enforcement Administration (DEA).