Cell Line Development

Our scientific experts have assisted in the development of a number of licensed biotherapeutic products and diagnostic reagents currently on the market today.

Our services enable you to maximize protein yield and establish economical production processes. Aragen Bioscience enhances existing systems that result in increased productivity and/or decreased production time. The scientists at Aragen will also help map out the most efficient development process allowing you to reach your goals faster. We have experience in many expression systems including Mammalian, Insect, Bacterial and Yeast.  In addition we have experience with a variety of unique mammalian gene expression systems including the Ubiquitous Chromatin Opening Element (UCOE™), Dihydrofolate Reductase (DHFR), Artificial Chromosome Element (ACE), Glutamine Synthetase (GS) and Chinese Hamster Elongation Factor (CHEF) technologies.

Our services include:

Molecular Biology and Cell Culture

• Molecular cloning of the gene of interest into a proprietary expression vector
• CDR grafting and chimeric antibody cloning
• Antibody isotype switching
• Transfection and selection of stable expression clonal cell lines
• Generation of serum-and protein-free suspension cell lines
• Cell banking

Cell Line Productivity and Stability Studies

• Cell specific productivity analysis
• Media optimization/testing
• Tissue culture and purification process development and optimization
• Generational stability of clones

Cell Culture

• Generation of serum-and protein-free suspension cell lines
• Cell banking
• Clone purification by subcloning

Analytical Services

• Restriction analysis
• Sequence confirmation
• Glycosylation analysis
• Southern blots, northern blots, western blots
• Cell proliferation and toxicity assays
• Kinase assays
• Immunohistochemistry/immunofluorescence
• Apoptosis assay
• ELISA flow cytometry
• ADCC/CDC
• Analytical method development of in-process and final product testing
• HPLC, ELISA, Western blot, SDS PAGE, IEF
• Binding assesment via Forte Bio Octet system